Free peptide calculator

Peptide reconstitution calculator

Calculate concentration and convert an amount in mcg into mL and U-100 syringe markings. Enter values from your product documentation or research method.

This tool checks the math. It cannot determine a suitable dose, verify vial contents, or choose a diluent. For a prescribed medicine, confirm the calculation and measuring device with your pharmacist.

Enter your values

Use the documented final volume, which may differ from the water added. The result assumes complete dissolution and uniform concentration.

Reconstitution formula and worked example

Total mcg = mg × 1,000. Concentration in mcg/mL = total mcg ÷ final mL. Volume in mL = requested mcg ÷ concentration. U-100 scale units = mL × 100.

For a math example, 5 mg in a final volume of 2 mL gives 2,500 mcg/mL. A 250 mcg portion occupies 0.1 mL, equivalent to 10 units on a U-100 scale. These example values are not a suggested preparation or dose.

mg-to-mcg reference

Milligram to microgram conversions
Milligrams (mg)Micrograms (mcg)
0.1100
0.25250
11,000
55,000
1010,000

Reconstitution questions

How does a peptide reconstitution calculator work?

It divides total peptide mass by final solution volume to find concentration, then divides a user-specified amount by that concentration to find volume. It does not choose a dose, diluent or preparation method.

How do I convert mg to mcg?

Multiply milligrams by 1,000. For example, 5 mg equals 5,000 mcg. This is a mass conversion; converting to mL also requires the solution concentration.

How many mL is 10 units on a U-100 syringe?

Ten U-100 scale units correspond to 0.1 mL, because that scale has 100 units per mL. The mass in that volume depends on concentration. Syringe markings are not peptide International Units (IU), and the conversion does not apply to a U-40 or other scale.

How much water should I add to a 5 mg or 10 mg vial?

The vial mass alone cannot determine an appropriate diluent or volume. Follow the exact product instructions or laboratory method. This calculator uses the final solution volume; the amount of water added can differ from final volume because of the formulation.

Can I convert peptide IU to mg with this calculator?

No. IU measure biological activity and require a substance-specific potency standard. They are different from the U-100 volume markings shown here.

How long does a reconstituted peptide last?

Use stability and handling instructions for the exact formulation and container. An opened diluent's discard date does not establish the chemical stability or sterility of a newly prepared solution. See our storage and water guides for the distinction.

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