How to Reconstitute BPC-157
Proper reconstitution of BPC-157 is critical for maintaining peptide integrity and ensuring accurate dosing. BPC-157 typically comes as a lyophilized powder in 5 mg vials. This guide walks through the complete process from supplies to storage.
Key Points
Step-by-Step Guide
Gather Supplies
You need: BPC-157 vial (typically 5 mg), bacteriostatic water, alcohol swabs, insulin syringe (1 mL), and a clean workspace. Have your dosing calculation ready.
Calculate Water Volume
For a 5 mg vial wanting 250 mcg per 0.1 mL (10 unit mark): add 2 mL of bacteriostatic water. Formula: Water (mL) = Peptide (mg) / Desired concentration (mg/mL). 5 mg / 2.5 mg/mL = 2 mL.
Sterilize Vial Tops
Swab both the BPC-157 vial and bacteriostatic water vial tops with alcohol. Let air dry for 10-15 seconds before puncturing.
Draw Bacteriostatic Water
Using a clean syringe, draw the calculated volume of BAC water. Remove air bubbles by tapping the syringe barrel and pushing air back into the vial.
Add Water to Peptide Vial
Insert needle into the BPC-157 vial at an angle. Aim the water stream at the glass wall, not directly onto the powder cake. Inject slowly and steadily.
Dissolve Gently
Let the vial sit 30-60 seconds, then gently swirl between your fingers. Never shake. The solution should become completely clear within 1-2 minutes.
Label and Store
Label with date, concentration, and peptide name. Store at 2-8°C (refrigerator). Use within 4 weeks. The solution must remain clear—discard if cloudy.
Warnings & Precautions
- !Never shake the vial—gentle swirling only.
- !Use bacteriostatic water, not sterile water, for multi-dose vials.
- !Discard if the solution is cloudy or contains particles.
- !Store reconstituted BPC-157 at 2-8°C; use within 28 days.
Frequently Asked Questions
How much bacteriostatic water for a 5 mg BPC-157 vial?
2 mL gives 250 mcg per 0.1 mL (10 units on insulin syringe). 1 mL gives 500 mcg per 0.1 mL. Choose based on your desired dose per injection.
How long does reconstituted BPC-157 last?
With bacteriostatic water, 4-6 weeks refrigerated. With sterile water (no preservative), use within 24-48 hours. Always use BAC water for multi-dose use.
What if BPC-157 does not dissolve?
Allow 2-3 minutes of gentle swirling. If still cloudy, refrigerate for 30 minutes and try again. Persistent cloudiness indicates degraded peptide—do not use.