Peptide Protocols
Step-by-step guides for peptide research. Dosing protocols, reconstitution instructions, and research summaries — all in one place.
50 protocols available
5-Amino-1MQ Dosing Protocol
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small molecule NNMT (nicotinamide N-methyltransferase) inhibitor studied for its effects on fat cell metabolism. By inhibiting NNMT, it increases NAD+ and SAM availability, potentially shifting fat cells from storage to energy expenditure.
View ProtocolAnti-Aging Peptide Cycle Guide
An anti-aging peptide cycle integrates multiple compounds targeting different aging hallmarks on staggered schedules. The key principle is that different peptides have different optimal cycling patterns, requiring careful scheduling to avoid overwhelm.
View ProtocolAnti-Aging Peptide Research Overview
Anti-aging peptides target specific hallmarks of aging: collagen decline, telomere shortening, cellular senescence, GH reduction, and mitochondrial dysfunction. No peptide has been proven to extend human lifespan, but several target measurable aging biomarkers.
View ProtocolAOD-9604 Dosing Protocol
AOD-9604 is a modified fragment of human growth hormone (amino acids 177-191) studied specifically for fat metabolism without the proliferative or diabetogenic effects of full-length GH. It has TGA approval in Australia as an oral supplement ingredient.
View ProtocolHow to Reconstitute AOD-9604
AOD-9604 reconstitution follows standard peptide protocols. Vials typically contain 5 mg. The microgram-range dosing (300-500 mcg) requires careful concentration planning for accurate daily administration.
View ProtocolBacteriostatic Water Guide
Bacteriostatic water (BAC water) is the foundation of peptide reconstitution. Containing 0.9% benzyl alcohol as a preservative, it allows reconstituted peptides to be stored for weeks while preventing bacterial growth. Understanding BAC water properties is essential for safe peptide handling.
View ProtocolBPC-157 Cycle Guide
Cycling BPC-157 appropriately maximizes healing potential while managing unknown long-term risks. Cycle length depends on the injury type, severity, and response. This guide covers cycle design from acute injuries to chronic conditions.
View ProtocolBPC-157 Dosing Protocol
BPC-157 (Body Protection Compound-157) is a pentadecapeptide derived from human gastric juice proteins. Research studies have explored dosing strategies across subcutaneous, intramuscular, and oral routes. This protocol compiles findings from published literature to guide research applications.
View ProtocolHow to Reconstitute BPC-157
Proper reconstitution of BPC-157 is critical for maintaining peptide integrity and ensuring accurate dosing. BPC-157 typically comes as a lyophilized powder in 5 mg vials. This guide walks through the complete process from supplies to storage.
View ProtocolBPC-157 + TB-500 Stack Dosing Protocol
The BPC-157 and TB-500 combination is the most popular healing peptide stack. BPC-157 provides targeted local tissue repair while TB-500 offers systemic wound healing support through complementary mechanisms.
View ProtocolCJC-1295 Dosing Protocol
CJC-1295 is a GHRH analog available in two forms: with DAC (Drug Affinity Complex) for extended half-life, and without DAC (Mod GRF 1-29) for pulsatile release. Understanding the difference is critical for protocol design.
View ProtocolHow to Reconstitute CJC-1295
CJC-1295 reconstitution is straightforward but you must know which version you have. The no-DAC version (Mod GRF 1-29) uses microgram dosing similar to Ipamorelin. The DAC version uses milligram dosing with different water volumes.
View ProtocolEpitalon Dosing Protocol
Epitalon (Epithalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) researched for telomerase activation. Based on epithalamin from the pineal gland, it targets cellular aging through telomere maintenance during brief periodic interventions.
View ProtocolHow to Reconstitute Epitalon
Epitalon reconstitution must account for its relatively high daily dose (5-10 mg) and short cycle duration (10-20 days). Planning your water volume for convenient daily draws over the full cycle minimizes vial handling and contamination risk.
View ProtocolFOXO4-DRI Dosing Protocol
FOXO4-DRI is a D-retro-inverso peptide designed to disrupt FOXO4-p53 interactions in senescent cells, triggering selective apoptosis. It represents one of the most targeted senolytic interventions studied, though human data is extremely limited.
View ProtocolGrowth Hormone Peptide Cycle Guide
GH peptide cycling balances the benefits of elevated growth hormone with the need to prevent pituitary desensitization and manage potential metabolic effects. Proper cycling includes on/off periods and regular monitoring.
View ProtocolGHK-Cu Dosing Protocol
GHK-Cu (copper peptide) is one of the most versatile peptides with applications from topical skincare to injectable tissue repair. As a naturally occurring tripeptide in human blood, it has one of the best-established safety profiles among research peptides.
View ProtocolHow to Reconstitute GHK-Cu
GHK-Cu reconstitution requires standard peptide handling with one additional consideration: the copper ion. While the copper complex is stable in solution, proper reconstitution preserves the peptide-copper bond essential for biological activity.
View ProtocolGLP-1 Receptor Agonist Research Overview
GLP-1 receptor agonists represent the most significant advance in obesity pharmacotherapy in decades. From semaglutide (single agonist) to tirzepatide (dual) to retatrutide (triple), each generation increases the number of metabolic pathways targeted.
View ProtocolGrowth Hormone Secretagogue Research Overview
Growth hormone secretagogues (GHS) stimulate the pituitary gland to release GH naturally, offering an alternative to exogenous GH injection. Two main classes exist: GHRH analogs (CJC-1295, Tesamorelin) and ghrelin mimetics (Ipamorelin, GHRP-2, GHRP-6, Hexarelin).
View ProtocolHealing Peptide Research Overview
Healing peptides represent a diverse group of compounds studied for tissue repair, wound healing, and recovery acceleration. BPC-157, TB-500, and GHK-Cu are the most researched, each targeting different aspects of the healing cascade.
View ProtocolHealing Stack Cycle Guide
The healing stack cycle combines BPC-157 and TB-500 in a structured timeline that accounts for TB-500 loading phase requirements, BPC-157 continuous dosing, and appropriate rest periods for injury reassessment.
View ProtocolHPLC Testing Explained
High Performance Liquid Chromatography (HPLC) is the standard method for measuring peptide purity. It separates the target peptide from impurities based on their chemical properties, quantifying the percentage of the sample that is the desired product.
View ProtocolIpamorelin + CJC-1295 Stack Dosing Protocol
The Ipamorelin and CJC-1295 no-DAC combination is the gold standard GH peptide stack. Combining a ghrelin mimetic (GHRP) with a GHRH analog produces synergistic GH release that is both stronger and longer-lasting than either peptide alone.
View ProtocolIpamorelin Dosing Protocol
Ipamorelin is a selective growth hormone secretagogue stimulating GH release without significantly affecting cortisol or prolactin. Its selectivity makes it one of the most studied GHRP-class peptides, with timing relative to meals and sleep being critical for GH pulse amplification.
View ProtocolHow to Reconstitute Ipamorelin
Ipamorelin requires precise reconstitution for accurate microgram-level dosing. Standard vials contain 2 mg or 5 mg. The water volume you choose determines how many units on the syringe equal your target dose.
View ProtocolMass Spectrometry for Peptide Analysis
Mass spectrometry (MS) is the gold standard for confirming peptide identity. By measuring the mass-to-charge ratio of ionized molecules, it confirms that a sample contains the expected peptide sequence with high precision.
View ProtocolMelanotan II Dosing Protocol
Melanotan II (MT-2) is a synthetic melanocortin receptor agonist researched for skin pigmentation, with additional effects on appetite and libido observed in studies. Protocols use a loading phase to initiate pigmentation followed by maintenance.
View ProtocolHow to Reconstitute Melanotan II
Melanotan II typically comes in 10 mg vials. Reconstitution is standard but the larger vial size and dose range (0.25-1.0 mg) require attention to concentration calculations for accurate dosing across loading and maintenance phases.
View ProtocolMOTS-c Dosing Protocol
MOTS-c is a mitochondrial-derived peptide encoded by 12S rRNA, studied for metabolic regulation, exercise mimetic properties, and cellular energy optimization. It activates AMPK signaling and improves insulin sensitivity in research models.
View ProtocolNootropic Peptide Research Review
Nootropic peptides target cognitive function through neurotrophic factor modulation, neuroprotection, and neurotransmitter system optimization. Semax and Selank are the most established, both approved for clinical use in Russia, providing a unique body of clinical data.
View ProtocolPeptide Research Basics
Peptides are short chains of amino acids that serve as signaling molecules in biological systems. Understanding their basic chemistry, mechanism of action, and the regulatory landscape is essential before engaging with any specific peptide protocol.
View ProtocolPeptide Stability Research Guide
Understanding peptide stability is critical for maintaining potency from purchase through administration. Peptides degrade through multiple pathways, and knowing which factors affect your specific peptide prevents waste and ensures consistent dosing.
View ProtocolPeptide Storage Protocol
Proper storage is the most overlooked aspect of peptide handling. Temperature, light, and moisture are the enemies of peptide stability. Understanding storage requirements preserves potency and prevents using degraded, potentially harmful products.
View ProtocolReconstitution Calculator Guide
Peptide dosing math intimidates many newcomers, but it reduces to one simple formula. Understanding the relationship between peptide amount, water volume, and syringe units eliminates dosing errors and builds confidence in peptide preparation.
View ProtocolRetatrutide Dosing Protocol
Retatrutide (LY3437943) is an investigational triple receptor agonist targeting GIP, GLP-1, and glucagon receptors simultaneously. Phase 2 trials demonstrated unprecedented weight loss of up to 24% at the highest dose, making it the most potent obesity treatment studied to date.
View ProtocolSelank Dosing Protocol
Selank is a synthetic tuftsin analog approved in Russia as an anxiolytic. It modulates GABA activity without the sedation, tolerance, or dependence associated with benzodiazepines, making it an attractive research option for anxiety management.
View ProtocolSemaglutide Dosing Protocol
Semaglutide is a GLP-1 receptor agonist studied for metabolic health and weight management. Its ~7-day half-life enables once-weekly dosing. Gradual titration is essential to minimize gastrointestinal side effects.
View ProtocolHow to Reconstitute Semaglutide
Compounded semaglutide comes as lyophilized powder requiring reconstitution, unlike the pre-filled pens of brand-name Ozempic/Wegovy. Proper reconstitution ensures accurate dosing across the titration schedule from 0.25 mg to 2.4 mg.
View ProtocolSemax Dosing Protocol
Semax is a synthetic ACTH(4-10) analog approved in Russia for cognitive impairment and stroke recovery. Available as a nasal spray, it offers a non-injection route for nootropic peptide research. Protocols derive from Russian clinical use and research community experience.
View ProtocolSterile Technique Guide
Sterile technique prevents bacterial contamination that can cause injection site infections or systemic illness. While home peptide handling cannot achieve clinical sterility, following basic aseptic principles dramatically reduces infection risk.
View ProtocolInsulin Syringe & Needle Guide
Choosing the right syringe is essential for comfortable, accurate peptide administration. Insulin syringes are the standard for subcutaneous peptide injections due to their fine gauge, precise markings, and appropriate volume range.
View ProtocolTB-500 Dosing Protocol
TB-500 (Thymosin Beta-4 fragment) is studied for tissue repair, cell migration, and anti-inflammatory activity. Protocols commonly use a loading phase followed by maintenance. This guide summarizes dosing approaches from published research.
View ProtocolHow to Reconstitute TB-500
TB-500 reconstitution follows the same principles as other peptides but uses larger dose volumes due to milligram-range dosing. Vials typically contain 5 mg or 10 mg. Proper calculation ensures accurate dosing during both loading and maintenance phases.
View ProtocolTesamorelin Dosing Protocol
Tesamorelin is an FDA-approved synthetic GHRH analog (Egrifta) indicated for HIV-associated lipodystrophy. It stimulates pulsatile GH release more physiologically than exogenous GH, making it a unique option in the growth hormone peptide landscape.
View ProtocolHow to Reconstitute Tesamorelin
Tesamorelin (Egrifta) comes with a manufacturer-provided diluent specifically designed for reconstitution. Unlike research peptides using BAC water, tesamorelin uses a proprietary sterile diluent, and the reconstituted solution should be used promptly.
View ProtocolTesofensine Dosing Protocol
Tesofensine is an oral triple monoamine reuptake inhibitor (serotonin, norepinephrine, dopamine) originally developed for neurological conditions and later studied for weight loss. Phase 2 trials showed significant weight reduction through appetite suppression and increased energy expenditure.
View ProtocolTirzepatide Dosing Protocol
Tirzepatide is a dual GIP/GLP-1 receptor agonist engaging both incretin pathways. The SURPASS and SURMOUNT trial programs demonstrated significant efficacy in glycemic control and weight reduction with structured titration.
View ProtocolUnderstanding Peptide Purity Testing
Peptide purity directly determines safety and efficacy. A peptide that is 95% pure contains 5% unknown impurities that could include failed synthesis sequences, chemical byproducts, or residual solvents. Understanding purity testing helps you evaluate vendor quality.
View ProtocolWeight Loss Peptide Cycle Guide
Weight loss peptide cycling differs from other peptide categories because GLP-1 agonists are typically used long-term rather than in traditional on/off cycles. However, strategic cycling of adjunct compounds alongside GLP-1 therapy can optimize outcomes.
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